Mouse Anti-E-Tag Recombinant Antibody (clone 11H12B3)
CAT#: ZG-0287U
Mouse anti-E-Tag antibody (clone 11H12B3) displayed high specificity against the E-Tag.
Tested Data
WB
Figure 1 Mouse Anti-E-Tag Antibody (ZG-0287U) in WB
Positive WB detected in: 1-3 lanes: E-tagged fusion protein, 4-5 lanes: Recombinant protein without E-tagged, 6 lane: BSA-E-Tag All lanes: E-Tag antibody at 1: 1000
Secondary
Goat polyclonal to Mouse IgG at 1/10000 dilution
Predicted band size: 30, 68, 55, 67-157kDa
Observed band size: 30, 68, 55, 67-157kDa
IP
Figure 2 Mouse Anti-E-Tag Antibody (ZG-0287U) in IP
Immunoprecipitating E-Tag in 293F transfected whole cell lysate Lane 1: Mouse control IgG (1µg) in 293F transfected whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)
Lane 2: ZG-0287U (5µg) + 293F transfected whole cell lysate (500µg)
Lane 3: 293F transfected whole cell lysate (20µg)
ELISA
Figure 3 Mouse Anti-E-Tag Antibody (ZG-0287U) in ELISA
ELISA E-Tag antibody in E-tagged fusion protein and Control protein (no E-tag) Antigen at dilution of 1µg/ml
E-Tag antibody at (0-0.15µg)
Goat polyclonal to Mouse IgG at 1/50000 dilution
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Specifications
- Immunogen
- GAPVPYPDPLEPRC synthetic peptide conjugate to KLH
- Host Species
- Mouse
- Type
- Mouse IgG1
- Specificity
- E-Tag
- Species Reactivity
- All
- Clone
- 11H12B3
- Applications
- ELISA, WB, IP
- Conjugate
- Unconjugated
Product Property
- Purification
- Protein G purified
- Purity
- >95% as determined by SDS-PAGE
- Buffer
- PBS, pH 7.4, 0.03% Proclin 300, 50% glycerol.
- Preservative
- 0.03% Proclin 300
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C for short term (1-2 weeks). Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- This antibody has been tested for use in Enzyme-linked Immunosorbent Assay, Western Blot, Immunoprecipitation. Specific conditions should be optimized by the end user.
ELISA:1:4000-1:8000, WB:1:1000-1:320000, IP:1µg-5µg
Target
- Alternative Names
- E-Tag
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Time-Saving
During our investigation, the accuracy and selectivity of this antibody allowed us to save a great deal of time. The outcomes from different assays were all consistent.
Highly Recommended
In our lab, this antibody is now a need for any connected endeavor. The dependability and quality are unparalleled.
Stable Over Time
We discovered that this antibody retained its stability and functionality after several usage and appropriate storage.
Q&As
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How do I prevent antibody degradation during storage?
A: Store the antibody at -20°C in aliquots, avoid repeated freeze-thaw cycles, and use stabilizing agents if necessary. Properly sealed containers also help to maintain stability.
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What are potential causes of weak signals in my assays?
A: Weak signals can be due to low antibody concentration, insufficient antigen presence, inadequate detection methods, or suboptimal assay conditions. Optimization may be required.
-
Can I use this antibody with automated staining systems?
A: Yes, many automated systems are compatible with this antibody, but it's crucial to verify its suitability according to the specific system and protocols used.
View the frequently asked questions answered by Creative Biolabs Support.
Cite This Product
To accurately reference this product in your publication, please use the following citation information:
(Creative Biolabs Cat# ZG-0287U, RRID: AB_3112066)
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
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